Do Y S, Kim E, Kim Y M
Department of Biology, College of Science, Yonset University, Seoul, Korea.
J Bacteriol. 1990 Mar;172(3):1267-70. doi: 10.1128/jb.172.3.1267-1270.1990.
Extracts of heterotrophically grown cells of Pseudomonas carboxydovorans were found to contain an inhibitor of carbon monoxide dehydrogenase (CO-DH). The inhibitor activity was not detected in CO-autotrophically grown cells. The inhibitor was extremely stable to heat treatment based on the extent of inhibition of CO-DH activity. The extent of inhibition was proportional to the amount of cell extract added to the reaction mixture. The inhibition was independent of a prior incubation period of the extracts with CO-DH. The inhibitor was precipitable with ammonium sulfate, phenol, and trichloroacetic acid. It was passed through benzoylated dialysis tubing and Amicon ultrafiltration membrane YM2. Denaturing and nondenturing polyacrylamide gel electrophoresis of CO-DH inactivated by inhibitor revealed that the mobilities of native enzyme and subunits were identical to those of active CO-DH. The inhibitor-treated CO-DH retained its original antigenic sites and exhibited enzyme activity upon activity staining. The CO-DH inhibitor of P. carboxydovorans was also active on CO-DHs from Pseudomonas carboxydohydrogena, Acinetobacter sp. strain JC1, and Pseudomonas carboxydoflava.
已发现食羧假单胞菌异养生长细胞的提取物含有一氧化碳脱氢酶（CO-DH）的抑制剂。在一氧化碳自养生长的细胞中未检测到该抑制剂活性。基于对CO-DH活性的抑制程度，该抑制剂对热处理极为稳定。抑制程度与添加到反应混合物中的细胞提取物量成正比。抑制作用与提取物与CO-DH的预孵育时间无关。该抑制剂可被硫酸铵、苯酚和三氯乙酸沉淀。它能透过苯甲酰化透析管和Amicon超滤膜YM2。经抑制剂灭活的CO-DH的变性和非变性聚丙烯酰胺凝胶电泳显示，天然酶和亚基的迁移率与活性CO-DH的迁移率相同。经抑制剂处理的CO-DH保留了其原始抗原位点，并在活性染色时表现出酶活性。食羧假单胞菌的CO-DH抑制剂对来自羧氢假单胞菌、不动杆菌属JC1菌株和食羧黄假单胞菌的CO-DH也有活性。
