Department of Family Medicine and Community Nursing, Medical University of Bialystok, Poland.
Neoplasma. 2011;58(1):45-50. doi: 10.4149/neo_2011_01_45.
It was demonstrated that TNF superfamily proteins may affect significantly the time of leukemic cells' survival in the course of B-cell chronic lymphocytic leukemia (B-CLL). The aim of our study was to evaluate the expression and release of BAFF (B-cell activating factor), APRIL (a proliferation-inducing ligand) and TRAIL (TNF-related apoptosis inducing ligand) molecules belonging to the cytokines of the superfamily of the tumor necrosis factor (TNF) by neutrophils (PMNs) and, for comparison, B cells isolated from the blood of patients with B-CLL vs. their concentration in the blood serum. 40 patients suffering from B-CLL and a control group of 15 healthy subjects were included in the study. Cytoplasmic fractions of PMNs and B cells were analyzed with the use of western blotting for the presence of TRAIL, BAFF and APRIL. Soluble TRAIL, BAFF and APRIL in the culture supernatants and the serum were assessed using ELISA kits. PMNs and B cells of patients with B-CLL before treatment demonstrated the statistically significantly higher expression of APRIL and BAFF proteins when compared with the control group of healthy subjects. In contrast, the expression of TRAIL protein in both types of cells of patients was statistically significantly lower than its expression in the control cells. In the supernatants of PMN and B lymphocytes of patients the decreased concentrations of sBAFF, unchanged of APRIL and increased of sTRAIL molecules were demonstrated. The results of studies carried out in patients with B-CLL before treatment indicate that the relations demonstrated between APRIL, BAFF and TRAIL molecules, released by neutrophils and B cells and relations between their concentrations in the serum can significantly influence the development of B-CLL.
研究表明，肿瘤坏死因子（TNF）超家族蛋白可能会显著影响 B 细胞慢性淋巴细胞白血病（B-CLL）中白血病细胞的存活时间。我们的研究目的是评估中性粒细胞（PMN）和 B 细胞（从 B-CLL 患者血液中分离）表达和释放 BAFF（B 细胞激活因子）、APRIL（增殖诱导配体）和 TRAIL（TNF 相关凋亡诱导配体）分子的情况，并比较它们在血清中的浓度。研究纳入了 40 名 B-CLL 患者和 15 名健康对照者。使用 Western blot 分析 PMN 和 B 细胞的细胞质部分中 TRAIL、BAFF 和 APRIL 的存在情况。使用 ELISA 试剂盒评估培养上清液和血清中可溶性 TRAIL、BAFF 和 APRIL 的浓度。与健康对照组相比，未经治疗的 B-CLL 患者的 PMN 和 B 细胞中 APRIL 和 BAFF 蛋白的表达水平显著更高。相比之下，两种细胞类型中 TRAIL 蛋白的表达水平均显著低于对照组。在患者的 PMN 和 B 淋巴细胞上清液中，sBAFF 浓度降低，APRIL 不变，sTRAIL 分子增加。在未经治疗的 B-CLL 患者中进行的研究结果表明，中性粒细胞和 B 细胞释放的 APRIL、BAFF 和 TRAIL 分子之间以及它们在血清中的浓度之间的关系可能会显著影响 B-CLL 的发展。
