Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX 77555, USA.
J Immunol. 2010 Dec 15;185(12):7174-9. doi: 10.4049/jimmunol.0903935. Epub 2010 Nov 10.
Severely burned patients were shown to be carriers of M2 monocytes, and all of the monocytes isolated from peripheral blood of severely burned patients (19 of 19 patients) were demonstrated as M2b monocytes (IL-12(-)IL-10(+)CCL1(+) monocytes). Low levels of M2a (IL-12(-)IL-10(+)CCL17(+) monocytes) and M2c monocytes (IL-12(-)IL-10(+)CXCL13(+) monocytes) were demonstrated in peripheral blood of severely burned patients (M2a, 2 of 19 patients; M2c, 5 of 19 patients). M2b, M2a, and M2c monocytes were not detected in peripheral blood of healthy donors. However, M2b monocytes appeared when healthy donor monocytes were cultured in media supplemented with burn patient serum (15%). CCL2 was detected in sera of all burn patients, and M2b monocytes were not generated from healthy donor monocytes cultured with media containing 15% burn patient sera that were previously treated with anti-CCL2 mAb. In addition, M2b monocytes were generated from healthy donor monocytes in cultures supplemented with rCCL2. These results indicate that M2b monocytes are predominant in peripheral blood of severely burned patients who are carriers of CCL2 that functions to stimulate monocyte conversion from resident monocytes to M2b monocytes.
严重烧伤患者被证明是 M2 单核细胞的携带者，从严重烧伤患者外周血中分离出的所有单核细胞（19 例患者中的 19 例）均被证明为 M2b 单核细胞（IL-12(-)IL-10(+)CCL1(+)单核细胞）。严重烧伤患者外周血中显示低水平的 M2a（IL-12(-)IL-10(+)CCL17(+)单核细胞）和 M2c 单核细胞（IL-12(-)IL-10(+)CXCL13(+)单核细胞）（M2a，19 例患者中的 2 例；M2c，19 例患者中的 5 例）。健康供体的外周血中未检测到 M2b、M2a 和 M2c 单核细胞。然而，当健康供体单核细胞在补充有烧伤患者血清（15%）的培养基中培养时，会出现 M2b 单核细胞。所有烧伤患者的血清中均检测到 CCL2，并且用含有 15%烧伤患者血清的培养基培养的健康供体单核细胞不会产生 M2b 单核细胞，该培养基先前用抗 CCL2 mAb 处理过。此外，在补充 rCCL2 的培养基中，M2b 单核细胞可从健康供体单核细胞中生成。这些结果表明，M2b 单核细胞在携带 CCL2 的严重烧伤患者外周血中占优势，CCL2 可作用于刺激常驻单核细胞向 M2b 单核细胞转化。
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