大鼠甲状腺素结合球蛋白（TBG）的肝脏生物合成：实验性甲状腺功能减退中的证实、个体发生及上调
The hepatic biosynthesis of rat thyroxine binding globulin (TBG): demonstration, ontogenesis, and up-regulation in experimental hypothyroidism.
作者信息
Vranckx R, Rouaze M, Savu L, Nunez E A, Beaumont C, Flink I L
机构信息
Unité INSERM 224, Faculté de Médecine Xavier Bichat, Paris, France.
出版信息
Biochem Biophys Res Commun. 1990 Feb 28;167(1):317-22. doi: 10.1016/0006-291x(90)91767-m.
Using a human thyroxine binding globulin (TBG) cDNA probe, we demonstrate that rat liver contains two TBG mRNA species of different length, consisting of about 1.8 Kb and 2.4 Kb respectively. Slot blot analysis of the hepatic mRNAs from rats of different age reveals a fair correlation between the developmental trend of the messengers and that of the TBG circulating levels. Finally Northern blot and slot studies demonstrate that the increase of serum TBG induced in adults by thyroidectomy actually reflects an enhanced hepatic biosynthesis of the protein.
利用人甲状腺素结合球蛋白（TBG）cDNA探针，我们证明大鼠肝脏含有两种长度不同的TBG mRNA，分别约为1.8 kb和2.4 kb。对不同年龄大鼠肝脏mRNA进行狭缝印迹分析，结果显示这些信使分子的发育趋势与TBG循环水平的发育趋势之间存在合理的相关性。最后，Northern印迹和狭缝印迹研究表明，甲状腺切除术后成年大鼠血清TBG的增加实际上反映了该蛋白肝脏生物合成的增强。
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