Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Nada-ku, Kobe, Hyogo, Japan.
Histochem Cell Biol. 2010 Dec;134(6):615-22. doi: 10.1007/s00418-010-0755-1. Epub 2010 Nov 11.
Small GTPases of the Rab family are key regulators of membrane trafficking. We produced antibodies against the Rab7 protein of Bombyx mori (BRab7) in rabbits, and against the Rab11 protein of B. mori (BRab11) in mice. The antibodies recognized BRab7 and BRab11 proteins, but did not recognize other Rab proteins. Immunoblotting of samples from brain tissue of B. mori revealed a single band for each antibody. Rab11 was expressed in most tissues, whereas Rab7 was expressed in the brain, ovary, and testis. Immunohistochemical reactivity of Rab7 and Rab11 in the brain of B. mori was restricted to neurons of the pars intercerebralis and dorsolateral protocerebrum. Double-labeling experiments demonstrated that immunohistochemical reactivity of Rab7 co-localized with that of Rab11 and partially with that of Rab8. Immunohistochemical reactivity of Rab11 and Rab8 co-localized with that of PERIOD, one of the proteins associated with circadian rhythm. These findings suggest that Rab7, Rab8, and Rab11 are involved in protein transport in the neurons of the brain of B. mori and might play a role in the control of circadian rhythm.
小 GTPases 家族的 Rab 是膜运输的关键调节因子。我们在兔子中产生了针对家蚕 Rab7 蛋白（BRab7）的抗体，在小鼠中产生了针对家蚕 Rab11 蛋白（BRab11）的抗体。这些抗体识别 BRab7 和 BRab11 蛋白，但不识别其他 Rab 蛋白。免疫印迹分析家蚕脑组织样本显示，每种抗体都只识别一个条带。Rab11 在大多数组织中表达，而 Rab7 则在脑、卵巢和睾丸中表达。在家蚕脑中，Rab7 和 Rab11 的免疫组织化学反应性仅限于中脑脑区和背外侧脑区的神经元。双标记实验表明，Rab7 的免疫组织化学反应性与 Rab11 和部分 Rab8 的反应性共定位。Rab11 和 Rab8 的免疫组织化学反应性与 PERIOD 共定位，PERIOD 是与生物钟相关的蛋白质之一。这些发现表明 Rab7、Rab8 和 Rab11 参与了家蚕脑神经元中的蛋白质运输，可能在生物钟控制中发挥作用。
Zotero 插件