Department of Marine Biology, Ocean University of China, Qingdao, 266003, China.
Mol Biol Rep. 2011 Apr;38(4):2273-9. doi: 10.1007/s11033-010-0358-x. Epub 2010 Oct 31.
Ikaros, an important transcription factor plays a role in the development of hemato-lymphoid system, yet its functional importance in fish macrophages remains unknown. In this study, an Ikaros cDNA was cloned from the half-smooth tongue sole Cynoglossus semilaevis. The cDNA contained an open reading frame of 1,290 nucleotides that encoded a 430 amino acid protein. The deduced protein is structurally similar to dul from other species, for example human, axolotl, and possesses 3-zinc finger and 2-zinc finger domains at its N- and C-termini, respectively. Phylogenetic analysis revealed C. semilaevis Ikaros to be grouped with all the fish Ikaros, but branching from other Ikaros family members. Both semi-quantitative PCR and quantitative real-time PCR indicated Ikaros to be predominantly expressed in the immune-relevant tissues such as kidney, thymus, spleen and liver. In the macrophages cultured from C. semilaevis head kidney and challenged with lipopolysaccharide and lipoteichoic acid not only induced expression of the proinflammatory cytokines tumor necrosis factor-alpha and interleukin 1-beta but also caused up-regulation of Ikaros in a dose- and time-dependent fashions. All these data suggest that Ikaros might be a useful marker for inflammatory responses in C. semilaevis.
Ikaros 是一种重要的转录因子，在造血淋巴系统发育中发挥作用，但它在鱼类巨噬细胞中的功能重要性尚不清楚。本研究从半滑舌鳎 Cynoglossus semilaevis 克隆了一个 Ikaros cDNA。cDNA 包含一个开放阅读框，长 1290 个核苷酸，编码一个 430 个氨基酸的蛋白质。推导出的蛋白质在结构上与其他物种的 dul 相似，例如人类、蝾螈，并在其 N-和 C-末端分别具有 3 个锌指和 2 个锌指结构域。系统发育分析表明，C. semilaevis Ikaros 与所有鱼类 Ikaros 聚为一组，但与其他 Ikaros 家族成员分支。半定量 PCR 和定量实时 PCR 均表明 Ikaros 主要在免疫相关组织中表达，如肾脏、胸腺、脾脏和肝脏。在从半滑舌鳎头肾培养的巨噬细胞中，用脂多糖和脂磷壁酸刺激后，不仅诱导了促炎细胞因子肿瘤坏死因子-α和白细胞介素 1-β的表达，而且还导致 Ikaros 在剂量和时间依赖性方式上调。所有这些数据表明，Ikaros 可能是半滑舌鳎炎症反应的一个有用标志物。
