Department of Physiological Chemistry, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan.
Dev Dyn. 2010 Dec;239(12):3416-35. doi: 10.1002/dvdy.22481.
The small GTPase Arf6 is a member of the Arf (ADP-ribosylation factor) family. Although the function of Arf6 has been heavily studied at the cellular level, its physiological function at the whole animal level is largely unknown. In this study, we examined both the tissue distribution and developmental timing of Arf6 expression in wild type mice to obtain valuable information to speculate on the physiological function of Arf6. Western blot analysis using anti-Arf6 antibody revealed that Arf6 was ubiquitously expressed with its developmental timing differing in a tissue-specific manner. These results were supported by Arf6 mRNA in situ hybridization experiments, which showed that Arf6 was highly expressed in the polarized epithelial cells and embryonic mesenchymal cells of most tissues in a temporally dependent manner. Taken in toto, our results suggest that the expression of Arf6 in mouse tissues is precisely regulated in a development- and tissue-dependent manner.
小 GTPase Arf6 是 Arf（ADP-核糖基化因子）家族的成员。尽管 Arf6 的功能在细胞水平上得到了深入研究，但它在整个动物水平上的生理功能在很大程度上仍是未知的。在这项研究中，我们检查了野生型小鼠中 Arf6 表达的组织分布和发育时间，以获得有价值的信息来推测 Arf6 的生理功能。使用抗 Arf6 抗体的 Western blot 分析表明，Arf6 广泛表达，其发育时间在组织特异性方面存在差异。这些结果得到了 Arf6 mRNA 原位杂交实验的支持，该实验表明，Arf6 在大多数组织的极化上皮细胞和胚胎间充质细胞中以时间依赖的方式高度表达。总的来说，我们的结果表明，Arf6 在小鼠组织中的表达以发育和组织依赖的方式被精确调控。
