Ophthalmology Department Laboratory of Nuclear Medicine, University Hospital of Larissa, Larissa, Greece.
Clin Exp Ophthalmol. 2011 May-Jun;39(4):324-9. doi: 10.1111/j.1442-9071.2010.02463.x. Epub 2011 Jan 14.
To compare aqueous humour and plasma levels of ghrelin, a peptide recently identified in human eyes, in patients with open-angle glaucoma and controls.
Cross-sectional, controlled, hospital-based study.
Twenty-four open-angle glaucoma (17 primary open-angle and 7 pseudo-exfoliation glaucoma) patients and 30 controls were included. All participants were patients scheduled for cataract or glaucoma surgery. Patients with other ocular pathology, previous ocular surgery or diabetes were excluded.
Blood samples were collected before elective surgery. Aqueous humour was aspirated from the anterior chamber through a paracentesis with a 27-G needle under sterile conditions before any tissue manipulation. Ghrelin quantification was performed with commercially available Radioimmunoassay kits.
Ghrelin levels in aqueous humour and plasma.
Plasma levels of ghrelin were 490.5 ± 156.0 pg/mL in the open-angle glaucoma and 482.2 ± 125.4 pg/mL in the control group (Mann-Whitney test, P = 0.897). Aqueous humour levels of ghrelin were 85.5 ± 15.4 and 123.4 ± 25.5 pg/mL in the respective groups (P < 0.001). The ratio of plasma/aqueous humour ghrelin concentration was higher in the open-angle glaucoma versus the control group (5.75 ± 1.92 vs. 4.00 ± 1.04, P < 0.001). There was no difference in aqueous humour levels of ghrelin between primary open-angle glaucoma and pseudo-exfoliation glaucoma patients (P = 0.494).
Aqueous humour levels of ghrelin were significantly lower in open-angle glaucoma patients, compared with controls. This difference may manifest a role of ghrelin in the disease process or a consequence of antiglaucoma treatment.
比较人眼内新发现的肽类物质——ghrelin 在开角型青光眼患者和对照者的房水和血浆中的浓度。
横断面、对照、医院为基础的研究。
24 例开角型青光眼（17 例原发性开角型和 7 例假性剥脱性青光眼）患者和 30 例对照者纳入本研究。所有患者均因白内障或青光眼手术而就诊。排除有其他眼部疾病、既往眼部手术或糖尿病史的患者。
所有患者均在择期手术前采集血样。在无菌条件下从前房通过 27-G 号针头进行房水抽吸，抽吸前不进行任何组织操作。应用商业可得的放射免疫试剂盒测定 ghrelin 浓度。
房水和血浆中的 ghrelin 浓度。
开角型青光眼组患者血浆 ghrelin 浓度为 490.5±156.0 pg/ml，对照组为 482.2±125.4 pg/ml（Mann-Whitney 检验，P=0.897）。房水中 ghrelin 浓度在两组患者分别为 85.5±15.4 和 123.4±25.5 pg/ml（P<0.001）。开角型青光眼组患者的血浆/房水 ghrelin 浓度比值明显高于对照组（5.75±1.92 比 4.00±1.04，P<0.001）。原发性开角型青光眼和假性剥脱性青光眼患者房水中 ghrelin 浓度无差异（P=0.494）。
与对照组相比，开角型青光眼患者房水中 ghrelin 浓度显著降低。这种差异可能提示 ghrelin 在疾病进程中的作用，或者是抗青光眼治疗的结果。
