O'Conner J L, Kellom T A
Department of Physiology and Endocrinology, Medical College of Georgia, Augusta 30912.
FEBS Lett. 1990 Feb 26;261(2):315-8. doi: 10.1016/0014-5793(90)80580-c.
Anterior pituitary cell cultures are frequently used in studying the control of gonadotropin secretion. Historically, many (if not most) of these studies have been performed in the presence of Phenol red as a pH indicator. Phenol red preparations, because of their potential estrogenic activity, may have influenced the results of previous studies defining the relative luteinizing hormone releasing hormone responsiveness of rat anterior pituitary-cells derived from various stages of the estrous cycle. We therefore felt it of interest to investigate this possibility by repeating our previous cycle-related superfusion studies [(1988) Life Sci. 42, 61-72] in the absence of these Phenol red preparations. Comparisons of data obtained in the presence or absence of Phenol red revealed cells derived from late proestrous (19.00) and cultured in the absence of Phenol red continued to evidence the highest LH responsiveness. However, diestrous 1 08.00 cells cultured in the absence of Phenol red were lower in responsiveness than previously observed in the presence of the substance and the responsiveness of proestrous 08.00 and 15.00 in the presence was lower in comparison to the same stages in the absence of Phenol red. The results suggest that Phenol red preparations are capable of modulating LHRH responsiveness in superfusion and that the effect is more pronounced at certain cycle stages than at others.
垂体前叶细胞培养常用于研究促性腺激素分泌的调控。从历史上看，许多（如果不是大多数）此类研究都是在酚红作为pH指示剂的情况下进行的。酚红制剂由于其潜在的雌激素活性，可能影响了先前确定源自发情周期不同阶段的大鼠垂体前叶细胞相对促黄体生成素释放激素反应性的研究结果。因此，我们认为有必要通过在没有这些酚红制剂的情况下重复我们之前与周期相关的灌流研究[（1988年）《生命科学》42卷，61 - 72页]来研究这种可能性。对在有或没有酚红的情况下获得的数据进行比较发现，源自发情前期晚期（19.00）且在没有酚红的情况下培养的细胞继续表现出最高的促黄体生成素反应性。然而，在没有酚红的情况下培养的动情期1（08.00）细胞的反应性低于之前在有该物质存在时观察到的情况，并且在有酚红存在时发情前期08.00和15.00阶段的反应性与在没有酚红时的相同阶段相比更低。结果表明，酚红制剂能够在灌流中调节促黄体生成素释放激素的反应性，并且这种效应在某些周期阶段比其他阶段更明显。
