由xyn10B基因编码的解凝乳芽孢杆菌细胞内木聚糖酶Xyn10B的特性分析。
Characterization of Paenibacillus curdlanolyticus intracellular xylanase Xyn10B encoded by the xyn10B gene.
作者信息
Sudo Maya, Sakka Makiko, Kimura Tetsuya, Ratanakhanokchai Khanok, Sakka Kazuo
机构信息
Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University, Tsu, Japan.
出版信息
Biosci Biotechnol Biochem. 2010;74(11):2358-60. doi: 10.1271/bbb.100555. Epub 2010 Nov 7.
The Paenibacillus curdlanolyticus xyn10B gene encoding a family-10 xylanase was cloned and expressed in Escherichia coli, and the recombinant enzyme rXyn10B was characterized. Immunological analysis suggested that Xyn10B is an intracellular enzyme. rXyn10B hydrolyzed birch-wood xylan and xylooligosaccharides to produce mainly xylobiose, suggesting that it is an endoxylanase. Its properties were significantly different from those of some homologous enzymes.
编码10族木聚糖酶的解凝胶芽孢杆菌木聚糖酶基因xyn10B在大肠杆菌中克隆并表达，对重组酶rXyn10B进行了表征。免疫学分析表明Xyn10B是一种细胞内酶。rXyn10B水解桦木木聚糖和木寡糖，主要产生木二糖，表明它是一种内切木聚糖酶。其性质与一些同源酶有显著差异。
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