Alshelmani Mohamed Idris, Loh Teck Chwen, Foo Hooi Ling, Lau Wei Hong, Sazili Awis Qurni
Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400 Serdang, Malaysia ; Department of Animal Production, Faculty of Agriculture, University of Sebha, Sebha, Libya.
Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400 Serdang, Malaysia ; Institute of Tropical Agriculture, Universiti Putra Malaysia (UPM), 43400 Serdang, Malaysia.
ScientificWorldJournal. 2014;2014:729852. doi: 10.1155/2014/729852. Epub 2014 Jun 12.
Four cellulolytic and hemicellulolytic bacterial cultures were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Culture (DSMZ) and the American Type Culture Collection (ATCC). Two experiments were conducted; the objective of the first experiment was to determine the optimum time period required for solid state fermentation (SSF) of palm kernel cake (PKC), whereas the objective of the second experiment was to investigate the effect of combinations of these cellulolytic and hemicellulolytic bacteria on the nutritive quality of the PKC. In the first experiment, the SSF was lasted for 12 days with inoculum size of 10% (v/w) on different PKC to moisture ratios. In the second experiment, fifteen combinations were created among the four microbes with one untreated PKC as a control. The SSF lasted for 9 days, and the samples were autoclaved, dried, and analyzed for proximate analysis. Results showed that bacterial cultures produced high enzymes activities at the 4th day of SSF, whereas their abilities to produce enzymes tended to be decreased to reach zero at the 8th day of SSF. Findings in the second experiment showed that hemicellulose and cellulose was significantly (P < 0.05) decreased, whereas the amount of reducing sugars were significantly (P < 0.05) increased in the fermented PKC (FPKC) compared with untreated PKC.
从莱布尼茨研究所DSMZ - 德国微生物和细胞培养物保藏中心（DSMZ）以及美国典型培养物保藏中心（ATCC）购买了四种纤维素分解和半纤维素分解细菌培养物。进行了两项实验；第一项实验的目的是确定棕榈仁粕（PKC）固态发酵（SSF）所需的最佳时间段，而第二项实验的目的是研究这些纤维素分解和半纤维素分解细菌的组合对PKC营养品质的影响。在第一项实验中，SSF持续12天，接种量为10%（v/w），针对不同的PKC与水分比。在第二项实验中，在四种微生物之间创建了十五种组合，并将一种未处理的PKC作为对照。SSF持续9天，对样品进行高压灭菌、干燥，并进行近似分析。结果表明，细菌培养物在SSF的第4天产生高酶活性，而它们产生酶的能力在SSF的第8天趋于下降至零。第二项实验的结果表明，与未处理的PKC相比，发酵后的PKC（FPKC）中的半纤维素和纤维素显著（P < 0.05）减少，而还原糖的量显著（P < 0.05）增加。
