Covidien, Imaging Solutions, 675 McDonnell Blvd., Hazelwood, MO 63042, USA.
Metallomics. 2010 Jul;2(7):490-7. doi: 10.1039/b915806e. Epub 2010 Jun 4.
The gadolinium species present in a rat kidney following intravenous administration of a gadolinium-based magnetic resonance contrast agent (Optimark™, Gadoversetamide injection) to a rat was examined in the present study. The major gadolinium species in the supernatant of the rat kidney tissue extracts was determined by reversed-phase liquid chromatography with online inductively coupled plasma optical emission spectrometry (HPLC-ICP-OES). The identity of the compound was established by liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) detection. The principal gadolinium(III) complex in a rat kidney tissue extract was identified as Gd-DTPA-BMEA 24 Hrs and 7 days after a single intravenous injection of Optimark™ (gadoversetamide; Gd-DTPA-BMEA) at a dose of 5 mmol Gd/kg body weight. The study demonstrated for the first time the feasibility of the use of two complementary techniques, HPLC-ICP-OES and HPLC-ESI-MS to study the in vivo behavior of gadolinium-based magnetic resonance contrast media.
本研究考察了大鼠静脉注射钆基磁共振对比剂（Optimark™，钆喷替酸葡甲胺注射液）后，大鼠肾脏中存在的钆物种。通过反相液相色谱与在线电感耦合等离子体发射光谱（HPLC-ICP-OES）测定了大鼠肾组织提取物上清液中的主要钆物种。通过液相色谱电喷雾电离质谱（LC-ESI-MS）检测确定了化合物的结构。在大鼠单次静脉注射 5mmol Gd/kg 体重的 Optimark™（gadosetamide；Gd-DTPA-BMEA）后 24 小时和 7 天，大鼠肾组织提取物中的主要钆（III）配合物被鉴定为 Gd-DTPA-BMEA。该研究首次证明了使用两种互补技术，HPLC-ICP-OES 和 HPLC-ESI-MS 研究基于钆的磁共振对比剂体内行为的可行性。
