Lassiter H A, Speranza M J, Hall R T, Meade V, Christensen R D, Parker C J
Division of Neonatology, University of Utah School of Medicine, Salt Lake City.
J Perinatol. 1990 Mar;10(1):27-31.
To determine the effect of intravenous immunoglobulin (IVIG) on the capacity of serum from septic neonates to deposit complement C3 and IgG onto the bacteria isolated from their blood, 500 mg/kg of IVIG was administered to 18 neonates suspected of being septic. Blood was obtained just before the infusion, and again 15 minutes after its completion. Group B streptococcus type II, group B streptococcus type III, Staphylococcus aureus, and Staphylococcus epidermidis were isolated from the pre-infusion blood of four neonates. Bacteria were incubated with the appropriate serum, washed, and the amount of C3 and IgG subsequently bound to the organisms was quantified by radioimmunobinding assay. Sera from the four septic neonates and sera from four neonates of similar gestational age but whose blood cultures were negative were compared with pooled sera from healthy adults. Before the administration of IVIG, C3 deposition onto the bacteria by sera from five of the seven neonates tested was significantly less than that observed for adult sera. Following the infusion, no increase in C3 deposition was observed for any of the seven sera assayed, and in two cases C3 deposition fell significantly. In contrast, in seven of eight cases, IVIG enhanced the IgG deposition to levels greater than or equal to those observed for adult sera. Therefore, following the infusion of IVIG into neonates with proven or suspected sepsis, the deposition of C3 onto invasive bacteria by their serum was not enhanced even though IgG deposition was increased.
为了确定静脉注射免疫球蛋白（IVIG）对脓毒症新生儿血清将补体C3和IgG沉积到从其血液中分离出的细菌上的能力的影响，对18名疑似脓毒症的新生儿给予500mg/kg的IVIG。在输注前和输注完成15分钟后再次采集血液。从4名新生儿输注前的血液中分离出II型B组链球菌、III型B组链球菌、金黄色葡萄球菌和表皮葡萄球菌。将细菌与适当的血清一起孵育，洗涤后，通过放射免疫结合测定法定量随后与生物体结合的C3和IgG的量。将4名脓毒症新生儿的血清以及4名胎龄相似但血培养阴性的新生儿的血清与健康成年人的混合血清进行比较。在给予IVIG之前，7名受试新生儿中5名的血清对细菌的C3沉积明显少于成人血清。输注后，7份检测血清中均未观察到C3沉积增加，且有2例C3沉积显著下降。相比之下，在8例中的7例中，IVIG将IgG沉积提高到高于或等于成人血清中观察到的水平。因此，在向已证实或疑似脓毒症的新生儿输注IVIG后，尽管IgG沉积增加，但其血清中C3在侵袭性细菌上的沉积并未增强。
