Tu Xiang, Su Ben-Sheng, Kong Yun-Hua, Zhu Jian-Rong
School of Environment, Beijing Normal University, Beijing 100875, China.
Huan Jing Ke Xue. 2010 Sep;31(9):2118-23.
The cultivation of aerobic granules in a large pilot-scale SBR was investigated using domestic wastewater. After operation of 210 days, the granules with a diameter of 330 microm were successfully formed by seeding anaerobic digested sludge. Results showed that, during the first three months of operation under low organic load of influent, removal efficiencies of pollutants increased steadily including COD, NH(4+)-N, total nitrogen (TN) and total phosphorous (TP). Meanwhile, microorganisms related to nitrogen and phosphorous were enriched. The organic load was enhanced since cycle time was shortened to 6 h, and the operational performance of the reactor still remained stable. Moreover, the average particle size of the sludge started to increase, along with excellent settling ability and high bioactivity. After the formation of aerobic granules, Sludge volume index (SVI) was maintained at 30 mL x g(-1) and the mixed liquor suspended solid (MLSS) concentration in the SBR reached 8.8 g x L(-1). MLVSS/MLSS ratio of the sludge increased to 82% with a high oxygen uptake rate (OUR) of 5.32 mg x (min x L)(-1). Bacillus was dominant on the outer layer of granules, while cocci were mainly located inside. The average COD and TP removal efficiencies kept at 90% and NH(4+) -N was almost completely depleted, NO(3-) -N and NO(2-) -N were not accumulated in the effluent. The aerobic granules were also capable of achieving simultaneous nitrification and denitrification (SND) in a single SBR cycle, which resulted in a high TN removal efficiency of 80%.
采用生活污水，在大型中试规模的序批式反应器（SBR）中研究了好氧颗粒的培养。运行210天后，通过接种厌氧消化污泥成功形成了直径为330微米的颗粒。结果表明，在进水有机负荷较低的运行前三个月，污染物的去除效率稳步提高，包括化学需氧量（COD）、铵态氮（NH(4+)-N）、总氮（TN）和总磷（TP）。同时，与氮和磷相关的微生物得到了富集。由于周期时间缩短至6小时，有机负荷提高，但反应器的运行性能仍保持稳定。此外，污泥的平均粒径开始增大，沉降性能优异，生物活性高。好氧颗粒形成后，污泥体积指数（SVI）维持在30 mL·g(-1)，SBR中的混合液悬浮固体（MLSS）浓度达到8.8 g·L(-1)。污泥的MLVSS/MLSS比值增至82%，氧摄取率（OUR）高达5.32 mg·(min·L)(-1)。芽孢杆菌在颗粒外层占主导地位，而球菌主要位于颗粒内部。平均COD和TP去除效率分别保持在90%，铵态氮几乎完全去除，出水未积累硝酸根氮（NO(3-)-N）和亚硝酸根氮（NO(2-)-N）。好氧颗粒还能够在单个SBR周期内实现同步硝化反硝化（SND），总氮去除效率高达80%。
