Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Epidemiol Infect. 2011 Aug;139(8):1230-8. doi: 10.1017/S0950268810002463. Epub 2010 Nov 15.
Rodents are a potential source of pathogenic Yersinia enterocolitica and Y. pseudotuberculosis. In order to study this, 190 rodents were captured and sampled on seven pig farms (n=110), five chicken farms (n=55) and six other locations (n=25) in Sweden. Pigs from three of the pig farms were also sampled (n=60). Pathogenic Y. enterocolitica was detected by TaqMan PCR in about 5% of rodent samples and 18% of pig samples. Only rodents caught on pig farms tested positive for the pathogen. Y. enterocolitica bioserotype 4/O:3 strains isolated from the rodent and pig samples were compared by pulsed-field gel electrophoresis and revealed a high degree of similarity, which was confirmed by random amplified polymorphic DNA. Y. pseudotuberculosis was only detected in one rodent sample. Thus, rodents may be vectors for the transmission of pathogenic Y. enterocolitica to pigs, acting as carriers rather than a reservoir, and should therefore remain an important issue in hygiene control measures on farms.
啮齿动物是致病性肠炎耶尔森菌和假结核耶尔森菌的潜在来源。为了研究这一点，在瑞典的七个猪圈（n=110）、五个鸡舍（n=55）和六个其他地点（n=25）捕获并采集了 190 只啮齿动物。还从三个猪圈中采集了猪的样本（n=60）。通过 TaqMan PCR 在约 5%的啮齿动物样本和 18%的猪样本中检测到了致病性肠炎耶尔森菌。只有在猪圈中捕获的啮齿动物对病原体检测呈阳性。从啮齿动物和猪样本中分离出的肠炎耶尔森菌生物血清型 4/O:3 菌株通过脉冲场凝胶电泳进行比较，结果显示高度相似，这通过随机扩增多态性 DNA 得到证实。假结核耶尔森菌仅在一个啮齿动物样本中被检测到。因此，啮齿动物可能是将致病性肠炎耶尔森菌传播给猪的媒介，充当载体而不是储主，因此在农场的卫生控制措施中仍然是一个重要问题。
