酵母酿酒酵母中 Cdc25p 磷酸化对 Ras2 鸟嘌呤核苷酸交换因子 (Ras2-GEF) 活性的反馈调节。
Feedback regulation of Ras2 guanine nucleotide exchange factor (Ras2-GEF) activity of Cdc25p by Cdc25p phosphorylation in the yeast Saccharomyces cerevisiae.
机构信息
Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Nankai District, Tianjin, PR China.
出版信息
FEBS Lett. 2010 Dec 1;584(23):4745-50. doi: 10.1016/j.febslet.2010.11.006. Epub 2010 Nov 10.
Ras-GEF Cdc25p has been found to be hyperphosphorylated upon glucose addition. This work provides evidence indicating that PKA activity positively regulates the degree of Cdc25p phosphorylation, and that the intracellular association of Cdc25p and Ras2p is independent of PKA activity. In vitro experiments revealed that the Ras2-GEF activity of Cdc25p is inhibited by Cdc25p phosphorylation. These data suggest a negative feedback mechanism by which intracellular cAMP synthesis is inhibited by PKA through Cdc25p phosphorylation.
当葡萄糖被加入时，Ras-GEF Cdc25p 被发现过度磷酸化。这项工作提供了证据表明 PKA 活性正向调节 Cdc25p 磷酸化的程度，并且 Cdc25p 和 Ras2p 的细胞内关联独立于 PKA 活性。体外实验表明 Cdc25p 的 Ras2-GEF 活性被 Cdc25p 磷酸化抑制。这些数据表明，通过 Cdc25p 磷酸化，细胞内 cAMP 合成被 PKA 抑制的负反馈机制。
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