National Dairy Research Institute, Karnal-132001, Haryana, India.
Res Vet Sci. 2012 Feb;92(1):53-9. doi: 10.1016/j.rvsc.2010.10.008. Epub 2010 Nov 13.
Retention of fetal membranes (RFM) is the major post-partum disorder in dairy cattle. Cyp19 gene encodes the aromatase enzyme responsible for catalyzing the rate limiting step in estrogen biosynthesis, an important hormone for placental maturation and expulsion. The present study was aimed for comparative analysis of Cyp19 gene expression and its epigenetic regulation in placental cotyledons of animals with and without RFM. Significantly lower expression of Cyp19 gene was found in placental samples of RFM affected animals in comparison to normal animals. Methylation analysis of 5 CpG dinucleotides of placenta specific Cyp19 gene promoter I.1 and proximal promoter, PII showed hypo-methylation of both PI.1 and PII in term placenta of normal and diseased animals. In conclusion, a mechanism other than promoter methylation is responsible for decreased aromatase expression in placental cotyledons of animals suffering from RFM.
胎衣不下（RFM）是奶牛产后主要的疾病。Cyp19 基因编码芳香酶，该酶负责催化雌激素生物合成的限速步骤，而雌激素是胎盘成熟和排出的重要激素。本研究旨在比较分析有和没有 RFM 的动物胎盘绒毛叶中 Cyp19 基因的表达及其表观遗传调控。与正常动物相比，患有 RFM 的动物的胎盘样本中 Cyp19 基因的表达显著降低。对胎盘特异性 Cyp19 基因启动子 I.1 和近端启动子 PII 的 5 个 CpG 二核苷酸进行甲基化分析显示，正常和患病动物的足月胎盘中，PI.1 和 PII 均呈低甲基化状态。综上所述，在患有 RFM 的动物的胎盘绒毛叶中，芳香酶表达降低的原因不是启动子甲基化。
