一种引发良好IgG抗体产生且IgE合成较少的白喉毒素-支链淀粉缀合物的制备。

Preparation of a diphtheria toxin-pullulan conjugate that elicits good IgG antibody production with poor IgE synthesis.

作者信息

Yamaya S, Yamamoto A, Komiya T, Mizuguchi J, Matuhasi T

机构信息

Department of Applied Immunology, National Institute of Health, Shinagawa-ku, Tokyo.

出版信息

Vaccine. 1990 Feb;8(1):65-9. doi: 10.1016/0264-410x(90)90180-t.

DOI:10.1016/0264-410x(90)90180-t

PMID:2107646

Abstract

Diphtheria toxin is detoxified through conjugation with pullulan. The toxin-pullulan conjugate is easily purified by DEAE-Sephacel chromatography. The conjugate forms a transparent 'clear line' with anti-toxin antibodies on agarose plate, which offers a good indicator of conjugate formation. The toxin-pullulan conjugate induces both IgG1 and IgG2b antibody production with diminished IgE response, while the alum-precipitated conventional toxoid causes mainly increases in IgE as well as IgG1 antibody formation. The anti-toxin HA titre (IgG antibody) induced by the toxin-pullulan conjugate parallels the neutralizing activity of the immune-sera. These results suggest that the conjugation of toxin to pullulan is a very powerful method by which to develop a vaccine that induces neutralizing antibody with diminished IgE antibody synthesis.

摘要

白喉毒素通过与支链淀粉结合而解毒。毒素-支链淀粉结合物可通过DEAE-葡聚糖凝胶层析轻松纯化。该结合物在琼脂糖平板上与抗毒素抗体形成透明的“清晰线条”，这是结合物形成的良好指标。毒素-支链淀粉结合物诱导产生IgG1和IgG2b抗体，同时降低IgE反应，而明矾沉淀的传统类毒素主要导致IgE以及IgG1抗体形成增加。毒素-支链淀粉结合物诱导的抗毒素HA滴度（IgG抗体）与免疫血清的中和活性平行。这些结果表明，毒素与支链淀粉的结合是一种非常有效的方法，可用于开发一种诱导中和抗体且减少IgE抗体合成的疫苗。