Agriculture and Agri-Food Canada Research Centre, 5403 - 1st Avenue South, Lethbridge, AB T1J 4B1, Canada.
Can J Microbiol. 2010 Nov;56(11):890-5. doi: 10.1139/w10-077.
Dried distillers' grain with solubles (DDGS) is a by-product of ethanol production, and its use as cattle feed has increased as a result of the expansion of the fuel ethanol industry. However, the inclusion of corn DDGS into feedlot diets may increase the shedding of Escherichia coli O157:H7. This study investigated whether corn or wheat DDGS at 2 concentrations (20% or 40% vs. 100% barley grain) affected the survival of E. coli O157:H7 in incubations of ruminal digesta and feces. Neither the type nor the level of DDGS had any effect on fermentation or the survival of E. coli O157:H7 in ruminal digesta. However, there was a time by DDGS interaction (p < 0.05), where the numbers of E. coli O157:H7 in feces did not differ after 4 or 12 h of incubation but were greater after 24 h in both 40% wheat and 40% corn DDGS as compared with other treatments. Additionally, after 24 h, the numbers of E. coli O157:H7 were greater in fecal incubations with corn DDGS than with wheat DDGS (p < 0.05). The differences in the numbers of E. coli O157:H7 were not attributable to changes in pH or in concentrations of volatile fatty acids in the media. These results suggest that the inclusion of high levels of corn or wheat DDGS in feedlot diets of cattle may encourage the survival of E. coli O157:H7 in feces.
干燥的酒糟可溶物（DDGS）是乙醇生产的副产品，随着燃料乙醇工业的扩张，其作为牛饲料的使用量增加。然而，玉米 DDGS 被纳入牛饲料可能会增加大肠杆菌 O157:H7 的脱落。本研究调查了玉米或小麦 DDGS 在 2 个浓度（20%或 40%比 100%大麦谷物）下是否会影响瘤胃消化物和粪便中大肠杆菌 O157:H7 的存活。DDGS 的类型和水平都不会影响发酵或瘤胃消化物中大肠杆菌 O157:H7 的存活。然而，存在 DDGS 时间交互作用（p<0.05），在 4 或 12 小时孵育后粪便中大肠杆菌 O157:H7 的数量没有差异，但在 40%小麦和 40%玉米 DDGS 处理下，24 小时后数量更大。此外，在 24 小时后，玉米 DDGS 粪便孵育中大肠杆菌 O157:H7 的数量大于小麦 DDGS（p<0.05）。大肠杆菌 O157:H7 数量的差异与培养基 pH 或挥发性脂肪酸浓度的变化无关。这些结果表明，在牛的饲养饲料中添加高水平的玉米或小麦 DDGS 可能会促进大肠杆菌 O157:H7 在粪便中的存活。
