接种大肠杆菌 O157:H7 并喂食含有可溶物的玉米或小麦酒糟的牛的粪便排泄。

Fecal shedding in cattle inoculated with Escherichia coli O157:H7 and fed corn or wheat distillers' dried grain with solubles.

机构信息

Department of Biological Sciences, University of Lethbridge, 4401 University Drive, Lethbridge, Alberta, Canada T1K 3M4.

出版信息

J Food Prot. 2013 Jan;76(1):114-8. doi: 10.4315/0362-028X.JFP-12-104.

DOI:10.4315/0362-028X.JFP-12-104

PMID:23317865

Abstract

Feeding corn dried distillers' grain with solubles (DDGS) has been linked to increased fecal shedding of Escherichia coli O157:H7 in cattle. A study was conducted to compare the impact of three diets containing (dry matter basis) 40% corn DDGS, 40% wheat DDGS, or 20% corn and 20% wheat mixed DDGS to a standard barley grain finishing diet on fecal shedding in cattle challenged with a 10(10) CFU mixture of four nalidixic acid-resistant E. coli O157:H7 strains. Rectal grab samples (n = 544) were collected over 70 days and screened for E. coli O157:H7 by direct plating and immunomagnetic bead separation. Feeding diets containing DDGS had no effect (P > 0.05) on the intensity or duration of fecal shedding of E. coli O157:H7 compared with the standard barley grain finishing diet.

摘要

用含有可溶物的干酒糟（DDGS）来喂养玉米，已被证明会增加牛粪便中大肠杆菌 O157:H7 的排泄量。本研究旨在比较三种日粮（以干物质基础计），即 40%玉米 DDGS、40%小麦 DDGS 或 20%玉米和 20%小麦混合 DDGS，与标准的大麦谷物育肥日粮对 10（10）CFU 四种耐萘啶酸的大肠杆菌 O157:H7 菌株混合后对牛粪便中大肠杆菌 O157:H7 排泄量的影响。在 70 天的时间里，采集了 544 个直肠抓取样本，并通过直接平板培养和免疫磁珠分离法来筛选大肠杆菌 O157:H7。与标准的大麦谷物育肥日粮相比，含有 DDGS 的日粮对大肠杆菌 O157:H7 的排泄强度或持续时间没有影响（P > 0.05）。