Millioni Renato, Miuzzo Manuela, Puricelli Lucia, Iori Elisabetta, Sbrignadello Stefano, Dosselli Ryan, Cecconi Daniela, Tessari Paolo, Righetti Pier Giorgio
Department of Clinical and Experimental Medicine, Chair of Metabolism, University of Padova, Padova, Italy.
Electrophoresis. 2010 Dec;31(23-24):3863-6. doi: 10.1002/elps.201000460.
Novel instrumentation for performing large-size (>25 cm) 2-D maps is reported here. To perform the first dimension, we developed a power supply that can deliver a voltage of up to 15,000 V and allows regulation of current (up to 200 μA) onto each individual focusing IPG strip. The IEF strip tray can accommodate up to 12 IPG strips and the electrodes slide on a ruler, thus permitting running strips of any length up to 45 cm. In addition, this apparatus also includes a second power supply that allows the performance of electrophoresis at high amperage (400 mA) and a Peltier system that allows a 10-80°C temperature control.
本文报道了用于制作大尺寸（>25厘米）二维图谱的新型仪器。为了进行第一维分析，我们开发了一种电源，它能够提供高达15000伏的电压，并允许调节施加到每个单独聚焦IPG条带上的电流（高达200微安）。IEF条带托盘最多可容纳12条IPG条带，电极可在标尺上滑动，从而能够运行长度达45厘米的任何条带。此外，该仪器还包括第二个电源，可实现高电流（400毫安）的电泳，以及一个珀耳帖系统，可实现10 - 80°C的温度控制。
