Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, 208016 Kanpur, India.
Macromol Biosci. 2011 Jan 10;11(1):22-35. doi: 10.1002/mabi.201000286.
In this study agarose-alginate scaffolds are synthesized using cryogelation technology in different formats like monolith, sheet, discs, and beads, and show amiable mechanical strength like soft tissue properties and high interconnected macroporous degradable architecture. In cell-material interactions, fibroblast (NIH-3T3) cells showed good adherence and proliferation on these scaffolds presenting its potential application in soft tissue engineering. The application of cryogel beads and monoliths was also examined by the efficient immobilization of bacterial cells (BL21) on these matrices revealing their use for recovery of product from continuous fermentation systems without cell leakage. These scaffolds also showed potential as a filter for repeated recovery of heavy metal binding, such as copper and nickel from the waste water. The cryogels prepared herein do have a number of unique features that make them an important class of soft materials for developing multi-featured scaffolds as a novel carrier for bioengineering applications.
在这项研究中，琼脂糖-海藻酸钠支架使用冷冻凝胶技术以不同的形式合成，如块状物、薄片、圆盘和珠粒，并具有类似软组织特性的宜人机械强度和高连通的大孔可降解结构。在细胞-材料相互作用中，成纤维细胞（NIH-3T3）细胞在这些支架上表现出良好的粘附和增殖，显示出其在软组织工程中的潜在应用。还通过将细菌细胞（BL21）有效地固定在这些基质上，研究了冷冻凝胶珠和块状物的应用，这表明它们可用于从连续发酵系统中回收产物而不会发生细胞泄漏。这些支架还可用作过滤器，可从废水中重复回收重金属结合物，如铜和镍。本文制备的冷冻凝胶具有许多独特的特点，使它们成为一类重要的软材料，可用于开发多功能支架，作为生物工程应用的新型载体。
