Solov'eva A I, Vysotskaia O N, Popov A S, Dolgikh Iu I
Izv Akad Nauk Ser Biol. 2010 Sep-Oct(5):574-80.
A cryopreservation method developed earlier was modified for freezing the calluses derived from mature embryos of four spring wheat (Triticum aestivum L.) pure lines. The effects of individual stages of cryopreservation protocol on the water content, number of calluses resuming growth, and rate of morphogenesis were analyzed. Growth resumption was observed in 90.5-100% of the calluses after dehydration and in 93.3-100% after freezing-thawing. The number of calluses displaying signs of morphogenesis considerably decreased after dehydration but remained almost the same after freezing-thawing.
对早前开发的一种冷冻保存方法进行了改良，用于冷冻4个春小麦（Triticum aestivum L.）纯系成熟胚诱导产生的愈伤组织。分析了冷冻保存方案各个阶段对水分含量、愈伤组织恢复生长的数量以及形态发生速率的影响。脱水后，90.5%-100%的愈伤组织恢复生长；冻融后，93.3%-100%的愈伤组织恢复生长。脱水后，出现形态发生迹象的愈伤组织数量大幅减少，但冻融后几乎保持不变。
