HeLa细胞中的抗坏血酸和脱氢抗坏血酸：它们对葡萄糖缺乏培养物中胶原肽酶活性的影响。
Ascorbic acid and dehydroascorbic acid in HeLa cells: their effect on the collagen-peptidase activity of glucose-deficient cultures.
作者信息
Boggust W A, McGauley H
出版信息
Br J Cancer. 1978 Jul;38(1):100-105. doi: 10.1038/bjc.1978.168.
HeLa cells in culture do not accumulate ascorbic acid unless ascorbic acid or dehydroascorbic acid is available in the medium. Collagen peptidase corresponding to the activity found in the invasive zone of tumours, and acid phosphatase, in HeLa cells cultured under normal conditions, are unaffected by ascorbic acid, but are reduced in cells deprived of carbohydrate. These reduced collagen-peptidase levels, but not acid phosphatase, are restored to the values of normal HeLa cells by ascorbic acid. The relevance of these findings is considered in the context of tumour growth and spread.
培养中的海拉细胞不会积累抗坏血酸，除非培养基中有抗坏血酸或脱氢抗坏血酸。在肿瘤侵袭区域发现的具有相应活性的胶原肽酶以及正常培养条件下的海拉细胞中的酸性磷酸酶，不受抗坏血酸的影响，但在缺乏碳水化合物的细胞中会减少。这些降低的胶原肽酶水平（而非酸性磷酸酶水平）通过抗坏血酸恢复到正常海拉细胞的值。在肿瘤生长和扩散的背景下考虑了这些发现的相关性。
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