Newworld Institute of Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, 130 Meilong Road, Shanghai 200237, China.
J Microbiol Methods. 2011 Jan;84(1):140-3. doi: 10.1016/j.mimet.2010.11.005. Epub 2010 Nov 13.
Four methods, namely enumeration of colony-forming units (CFU), aerobic respiration, MTT reduction capacity and succinate dehydrogenase activity were compared to determine the viability of E. coli cells at the early and late exponential growth phases. Our results revealed that great differences in cell viability existed between these methods and that the choice of method to determine cell viability must be made with caution.
四种方法，即集落形成单位（CFU）计数、需氧呼吸、MTT 还原能力和琥珀酸脱氢酶活性，被用来比较确定处于早期和晚期指数生长阶段的大肠杆菌细胞的活力。我们的结果表明，这些方法之间存在着细胞活力的巨大差异，因此必须谨慎选择确定细胞活力的方法。
