Azienda Ospedaliero-Universitaria Careggi and University of Florence, Florence, Italy.
Ann Intern Med. 2010 Nov 16;153(10):650-4. doi: 10.7326/0003-4819-153-10-201011160-00007.
Mesenchymal stem cells can differentiate into endothelial cells and participate in angiogenesis in adults. In experimental models of acute myocardial infarction, mesenchymal stem cells led to the recovery of cardiac function through the formation of a new vascular network.
To describe treatment with intravenous infusions of expanded autologous mesenchymal stem cells in 1 patient with critical limb ischemia due to systemic sclerosis.
Case report.
The rheumatology unit at the University of Florence, Florence, Italy.
A woman, aged 34 years, with systemic sclerosis who developed acute gangrene of the upper and lower limbs.
3 intravenous pulses of expanded autologous mesenchymal stem cells.
Angiography, skin histopathology, and immunohistochemistry.
Areas of necrotic skin were reduced after the first mesenchymal stem-cell infusion. After the third infusion, angiography showed revascularization of the patient's extremities. Skin section analysis revealed cell clusters with tubelike structures, and angiogenic factors were strongly expressed.
Causality cannot be established by a single case.
In patients with systemic sclerosis who have severe peripheral ischemia, intravenous infusion of expanded autologous mesenchymal stem cells may foster the recovery of the vascular network, restore blood flow, and reduce skin necrosis.
Fondazione Cassa di Risparmio di Pistoia e Pescia (partial funding).
间充质干细胞可以分化为内皮细胞，并在成体中参与血管生成。在急性心肌梗死的实验模型中，间充质干细胞通过形成新的血管网络导致心脏功能的恢复。
描述对 1 例因系统性硬化症导致严重肢体缺血的患者进行静脉输注扩增自体间充质干细胞的治疗情况。
病例报告。
意大利佛罗伦萨大学风湿病科。
1 例 34 岁女性，患有系统性硬化症，出现上下肢急性坏疽。
3 次静脉输注扩增自体间充质干细胞。
血管造影、皮肤组织病理学和免疫组织化学。
第 1 次间充质干细胞输注后，坏死皮肤面积减少。第 3 次输注后，血管造影显示患者四肢的血管再通。皮肤切片分析显示细胞簇具有管状结构，血管生成因子强烈表达。
单例病例不能确定因果关系。
在患有严重外周缺血的系统性硬化症患者中，静脉输注扩增自体间充质干细胞可能有助于恢复血管网络，恢复血流并减少皮肤坏死。
皮斯托亚和佩斯卡拉储蓄银行基金会（部分资助）。
