在气相色谱-质谱分析之前，减少从生物样品中纯化2-花生四烯酸甘油酯过程中的酰基迁移。
Reducing acyl migration during purification of 2-arachidonoylglycerol from biological samples before gas chromatography mass spectrometry analysis.
作者信息
Higuchi Sei, Irie Keiichi, Nakano Takafumi, Sakamoto Yuya, Akitake Yoshiharu, Araki Maiko, Ohji Makiko, Furuta Riyo, Katsuki Mai, Yamaguchi Ryuji, Matsuyama Kiyoshi, Mishima Kenji, Mishima Kenichi, Iwasaki Katsunori, Fujiwara Michihiro
机构信息
Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Fukuoka 814–0180, Japan.
出版信息
Anal Sci. 2010;26(11):1199-202. doi: 10.2116/analsci.26.1199.
Endocannabinoid 2-arachidonoylglycerol (2-AG) regulates several important physiological processes in the brain. 2-AG is commonly quantified by gas chromatography mass spectrometry after an initial purification step. The most precise and rapid purification utilizes C(18) solid-phase extraction, but quantification problems can arise with acyl migration from 2-AG to 1-arachidonoylglycerol. We found that extraction with methanol promoted this migration, but acetone and diethyl ether (Et(2)O) did not. Acetone and Et(2)O were used to develop a purification method for the direct determination of 2-AG.
内源性大麻素2-花生四烯酸甘油酯（2-AG）调节大脑中的几个重要生理过程。2-AG通常在初始纯化步骤后通过气相色谱质谱法进行定量。最精确和快速的纯化方法是使用C(18)固相萃取，但由于酰基从2-AG迁移到1-花生四烯酸甘油酯，可能会出现定量问题。我们发现用甲醇萃取会促进这种迁移，但丙酮和二乙醚（Et(2)O）不会。使用丙酮和Et(2)O开发了一种直接测定2-AG的纯化方法。
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