Ha Yun-Sok, Yan Chunri, Park Cheol, Yun Seok-Joong, Moon Sung-Kwon, Choi Yung Hyun, Kim Wun-Jae
Department of Urology, College of Medicine, Chungbuk National University, Cheongju, South Korea.
Urol Int. 2011;86(1):41-6. doi: 10.1159/000321689. Epub 2010 Nov 16.
Glutathione S-transferases have been implicated in the development of bladder cancer (BC). We investigated the genotype and expression of glutathione S-transferase-mu (GSTM1) and glutathione S-transferase-theta (GSTT1) in BC tissue specimens.
Tumor samples and matched normal mucosae were obtained from 34 patients. Genomic DNA was used to analyze GSTM1 and GSTT1 genotypes using multiplex polymerase chain reaction. GSTM1 and GSTT1 mRNA levels were measured using real-time reverse transcriptase polymerase chain reaction.
GSTM1 mRNA expression was lower in tumor tissues than in matched normal bladder mucosae, whereas GSTT1 mRNA expression was significantly higher. GSTT1 mRNA expression was higher in muscle-invasive BC and high-grade cancers than in non-muscle-invasive BC and lower-grade tumors.
GSTT1 is correlated with characteristics of aggressive BC. GSTT1 may play an important role in tumorigenesis and disease progression in patients with BC.
谷胱甘肽S-转移酶与膀胱癌（BC）的发生发展有关。我们研究了膀胱癌组织标本中谷胱甘肽S-转移酶-μ（GSTM1）和谷胱甘肽S-转移酶-θ（GSTT1）的基因型和表达情况。
从34例患者获取肿瘤样本及配对的正常黏膜组织。采用多重聚合酶链反应，利用基因组DNA分析GSTM1和GSTT1基因型。使用实时逆转录聚合酶链反应检测GSTM1和GSTT1 mRNA水平。
肿瘤组织中GSTM1 mRNA表达低于配对的正常膀胱黏膜组织，而GSTT1 mRNA表达显著更高。GSTT1 mRNA在肌层浸润性膀胱癌和高级别癌中的表达高于非肌层浸润性膀胱癌和低级别肿瘤。
GSTT1与侵袭性膀胱癌的特征相关。GSTT1可能在膀胱癌患者的肿瘤发生和疾病进展中起重要作用。
