Kim S K, Kaiser D
Department of Biochemistry, Stanford University School of Medicine, California 94305.
Cell. 1990 Apr 6;61(1):19-26. doi: 10.1016/0092-8674(90)90211-v.
During fruiting body development, the product of the csgA gene is necessary for cellular aggregation, for spore differentiation, and for gene expression that is initiated after 6 hr of starvation. From nascent wild-type fruiting bodies we have purified a polypeptide of 17 kd called C-factor, which, at approximately 1 to 2 nM, restores normal development to csgA mutant cells. C-factor activity is not recovered from extracts of unstarved, growing cells or csgA mutant cells. The amino acid sequence from purified C-factor demonstrates that it is the product of the csgA gene. C-factor is active over a narrow range of concentration and has properties of a morphogenetic paracrine signal.
在子实体发育过程中，csgA基因的产物对于细胞聚集、孢子分化以及饥饿6小时后启动的基因表达是必需的。我们从新生的野生型子实体中纯化出一种17kd的多肽，称为C因子，在大约1至2 nM的浓度下，它能使csgA突变细胞恢复正常发育。未饥饿的生长细胞或csgA突变细胞的提取物中无法恢复C因子活性。纯化的C因子的氨基酸序列表明它是csgA基因的产物。C因子在狭窄的浓度范围内具有活性，具有形态发生旁分泌信号的特性。
