Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706-1544, USA.
Chem Commun (Camb). 2011 Jan 21;47(3):973-5. doi: 10.1039/c0cc03864d. Epub 2010 Nov 16.
The S-peptide and S-protein components of bovine pancreatic ribonuclease form a noncovalent complex with restored ribonucleolytic activity. Although this archetypal protein-fragment complementation system has been the object of historic work in protein chemistry, intrinsic limitations compromise its utility. Modern methods are shown to overcome those limitations and enable new applications.
牛胰核糖核酸酶的 S-肽和 S-蛋白组份形成具有恢复的核糖核酸酶活性的非共价复合物。尽管这种典型的蛋白质片段互补系统一直是蛋白质化学历史研究的对象，但内在的局限性限制了其用途。现代方法被证明能够克服这些局限性并实现新的应用。
