Taizhou Municipal Hospital, 318000 Taizhou, Zhejiang, China.
Med Oncol. 2011 Dec;28 Suppl 1:S553-9. doi: 10.1007/s12032-010-9740-8. Epub 2010 Nov 16.
To study the serum protein fingerprint of patients with cervical cancer and to screen for protein molecules closely related to cervical cancer during the onset and progression of the disease using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Serum samples from 85 patients with cervical cancer and 80 healthy volunteers. Weak cation exchange (WCX) magnetic beads and PBSII-C protein chips reader (Ciphergen Biosystems Ins.) were used.The protein fingerprint expression of all the serum samples and the resulting profiles between cancer and normal were analyzed with Biomarker Wizard system. A group of proteomic peaks were detected. Three differently expressed potential biomarkers were identified with the relative molecular weights of 3974 Da, 4175 Da, 5906 Da. This diagnostic model can distinguish cervical cancer from healthy controls with a sensitivity of 93.3% and a specificity of 95%. Blind test data indicated a sensitivity of 87.5% and a specificity of 90%. MALDI technology can be used to screen significant proteins of differential expression in the serum of cervical cancer patients. These different proteins could be specific biomarkers of the patients with cervical cancer in the serum and have the potential value of further investigation.
采用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）技术，研究宫颈癌患者血清蛋白质指纹图谱，筛选与宫颈癌发生、发展密切相关的蛋白质分子。收集 85 例宫颈癌患者和 80 例健康志愿者的血清标本。应用弱阳离子交换（WCX）磁珠和 PBSII-C 蛋白质芯片阅读仪（Ciphergen Biosystems Ins.）。应用 Biomarker Wizard 系统对所有血清标本的蛋白质指纹图谱表达及癌与正常组间的差异图谱进行分析。检测到一组蛋白质峰，其中相对分子质量为 3974 Da、4175 Da、5906 Da 的 3 个差异表达的潜在生物标志物被鉴定。该诊断模型对宫颈癌与健康对照的鉴别具有 93.3%的敏感性和 95%的特异性。盲法测试数据显示敏感性为 87.5%，特异性为 90%。MALDI 技术可用于筛选宫颈癌患者血清中差异表达的显著蛋白质，这些不同的蛋白质可能是宫颈癌患者血清中的特异性生物标志物，具有进一步研究的潜在价值。
