van Dijk Ko Willems, Kypreos Kyriakos E, Fallaux Frits J, Hageman Jurre
Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands.
Methods Mol Biol. 2011;693:321-43. doi: 10.1007/978-1-60761-974-1_20.
Recombinant adenoviruses are attractive vectors for short-term expression in mouse liver and primary cell lines. Various versatile vector systems have been developed which can be used for the reliable production of recombinant adenoviruses. This protocol describes the entire process for the production of recombinant adenoviruses using the AdEasy system. This protocol will give a practical step-by-step description from the cloning of the gene of interest until the in vivo administration in mice. The entire process will take about 8 weeks to complete.
重组腺病毒是用于在小鼠肝脏和原代细胞系中进行短期表达的有吸引力的载体。已经开发了各种通用载体系统，可用于可靠地生产重组腺病毒。本方案描述了使用AdEasy系统生产重组腺病毒的整个过程。本方案将提供从感兴趣基因的克隆到在小鼠体内给药的实际分步描述。整个过程大约需要8周才能完成。
