Department of Kinesiology and Physical Education, Faculty of Education, McGill University, Canada.
Biochem Biophys Res Commun. 2010 Dec 17;403(3-4):351-6. doi: 10.1016/j.bbrc.2010.11.034. Epub 2010 Nov 23.
In this study, we show a method for direct measurements of force and simultaneous visualization of isolated muscle filaments. Single actin filaments isolated from chicken skeletal muscle and single thick filaments isolated from Mussels were imaged using fluorescence and dark field microscopy, respectively. Force generated by the filaments was measured using micro-fabricated cantilevers. Force values were in the range observed previously with myosin filaments and molecules. The results suggest that the technique can be used to investigate many issues of interest and debate in the field of muscle biophysics.
在这项研究中，我们展示了一种直接测量力和同时可视化分离肌肉纤维丝的方法。使用荧光和暗场显微镜分别对从鸡骨骼肌中分离的单个肌动蛋白纤维丝和从贻贝中分离的单个粗肌丝进行成像。使用微制造的悬臂梁测量纤维丝产生的力。所测量的力值处于先前用肌球蛋白纤维丝和分子观察到的范围内。结果表明，该技术可用于研究肌肉生物物理学领域的许多感兴趣和有争议的问题。
