在正常和肿瘤细胞中检测到人类线粒体转录因子 A 的新同工型。

New isoforms of human mitochondrial transcription factor A detected in normal and tumoral cells.

机构信息

Department of Biochemistry and Molecular Biology Ernesto Quagliariello, University of Bari, Bari, Italy.

出版信息

Mitochondrion. 2011 Mar;11(2):287-95. doi: 10.1016/j.mito.2010.10.009. Epub 2010 Nov 19.

DOI:10.1016/j.mito.2010.10.009

Abstract

Novel alternatively spliced variants of the human mitochondrial transcription factor A predicted by the computational tool ASPic were experimentally validated in different normal and tumoral human tissues by RT-PCR and DNA sequencing. The comparison between the 5'UTR length and the distribution of the different transcripts showed that the transcripts with the shortest 5'UTR are present in all the investigated tissues, while the longest 5'UTR seems to be related to tissue-specificity. Studies about the localization and function of the most widely diffuse alternative isoform Tr6 were carried out.

摘要

通过计算工具 ASPic 预测的人类线粒体转录因子 A 的新型剪接变体，通过 RT-PCR 和 DNA 测序在不同的正常和肿瘤人类组织中进行了实验验证。5'UTR 长度和不同转录本分布的比较表明，所有研究组织中都存在最短 5'UTR 的转录本，而最长 5'UTR 似乎与组织特异性有关。对最广泛分布的替代异构体 Tr6 的定位和功能进行了研究。