College of Sciences, Northeastern University, Shenyang 110004, China.
Biosens Bioelectron. 2011 Jan 15;26(5):2442-7. doi: 10.1016/j.bios.2010.10.029. Epub 2010 Oct 23.
We herein report a folding-based electrochemical DNA aptasensor for the detection of vascular endothelial growth factor (VEGF) directly in complex biological samples, including blood serum and whole blood. The electrochemical signal generation is coupled to a large, target-induced conformational change in a methylene blue-modified and surface immobilized anti-VEGF aptamer. The sensor is sensitive, selective and essentially reagentless: we can readily detect VEGF down to 5 pM (190 pg/mL) directly in 50% blood serum. Similar to other aptasensors of this class, the VEGF sensor is also regenerable and reusable. In addition, the sensor performs comparably well even when fabricated on a gold-plated screen-printed carbon electrode and can potentially be implemented as a cost-effective, single-use biosensor for diseases diagnosis and therapy monitoring. The exceptional sensitivity, selectivity, and reusability of this electrochemical aptasensor platform suggest it may be a promising strategy for a wide variety of sensing applications.
我们在此报告了一种基于折叠的电化学 DNA 适体传感器，可直接在包括血清和全血在内的复杂生物样品中检测血管内皮生长因子（VEGF）。电化学信号的产生与甲基蓝修饰和表面固定的抗 VEGF 适体的大的、靶诱导的构象变化偶联。该传感器具有灵敏度高、选择性好、基本无需试剂等特点：我们可以在 50%的血清中直接检测到低至 5 pM（190 pg/mL）的 VEGF。与该类其他适体传感器类似，VEGF 传感器也可重复使用和再生。此外，即使在镀金的丝网印刷碳电极上制备，传感器的性能也相当好，并且有可能作为一种用于疾病诊断和治疗监测的经济高效、一次性生物传感器。这种电化学适体传感器平台具有出色的灵敏度、选择性和可重复性，表明它可能是各种传感应用的有前途的策略。
