Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland.
Chair of Drug and Cosmetics Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Koszykowa 75, 00-664 Warsaw, Poland.
Biosensors (Basel). 2024 Feb 19;14(2):110. doi: 10.3390/bios14020110.
Lead poses severe effects on living organisms, and since Pb ions tend to accumulate in different organs, it is crucial to monitor Pb concentration in samples such as water and soil. One of the approaches is the utilization of biosensors combined with aptamer-based layers for the electrochemical detection of lead ions. Herein, we present the studies of applying miniaturized screen-printed transducers as solid surfaces to fabricate aptamer layers. As the research is the direct continuation of our previous studies regarding the use of gold disk electrodes, the working parameters of elaborated aptasensors were defined, including the range of linear response (10-100 nM), selectivity as well as stability, regeneration, and feasibility of application for the analysis of real samples. This was achieved using voltammetric techniques including cyclic and square-wave voltammetry in the presence of methylene blue redox indicator.
铅对生物体有严重的影响，而且由于 Pb 离子容易在不同的器官中积累，因此监测水样和土样中的 Pb 浓度至关重要。其中一种方法是利用生物传感器与基于适配体的层结合，用于电化学检测 Pb 离子。在这里，我们提出了利用微型丝网印刷换能器作为固体表面来制备适配体层的研究。由于这项研究是我们之前关于使用金盘电极的研究的直接延续，因此确定了精心设计的适配体传感器的工作参数，包括线性响应范围（10-100 nM）、选择性以及稳定性、再生性和应用于实际样品分析的可行性。这是通过使用包括循环伏安法和方波伏安法在内的伏安技术，在亚甲蓝氧化还原指示剂的存在下实现的。
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