Institute of Cancer Research, Male Urological Cancer Research Centre, Sutton, UK.
J Clin Pathol. 2011 Jan;64(1):88-90. doi: 10.1136/jcp.2010.082339. Epub 2010 Nov 15.
Formalin-fixed prostate biopsies are frequently the only tissue collected at the time of prostate cancer diagnosis. There is therefore a requirement for techniques that allow the use of these prostate biopsy specimens in a high-throughput analysis of immunohistochemical and fluorescence-in-situ-hybridisation-detected biomarkers.
The authors have previously described methods that allow tissue microarray (TMA) construction from prostate biopsies. Here, we describe significant technical innovations that provide an easier and more robust system of biopsy-TMA construction.
The TMAs produced are of a high density (up to 104 cores each, 8 × 13) and allow a multiplex analysis of biomarkers in the context of clinical trials.
在诊断前列腺癌时,福尔马林固定的前列腺活检通常是唯一采集的组织。因此,需要有技术能够在高通量分析免疫组织化学和荧光原位杂交检测到的生物标志物时,利用这些前列腺活检标本。
作者之前已经描述了从前列腺活检中构建组织微阵列(TMA)的方法。在这里,我们描述了一些重要的技术创新,这些创新提供了一种更简单、更强大的活检-TMA 构建系统。
生成的 TMA 密度很高(每个可达 104 个核心,8×13),并且允许在临床试验中对生物标志物进行多重分析。