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Inhibition of T4 polynucleotide kinase by the ATP analog, beta, gamma-imidoadenylyl 5'-triphosphate.

作者信息

Lillehaug J R

出版信息

Biochim Biophys Acta. 1978 Aug 7;525(2):357-63. doi: 10.1016/0005-2744(78)90230-9.

DOI:10.1016/0005-2744(78)90230-9
PMID:210816
Abstract

The inhibition of T4 polynucleotide kinase by beta,gamma-imidoadenylyl 5'-triphosphate has been investigated. It was found that the ATP analog was a competitive inhibitor with regard to ATP and a noncompetitive inhibitor with regard to DNA possessing a 5'-hydroxyl group. At pH 8.0, the Ki values were 3 and 11 mM, respectively. beta,gamma-imidoadenylyl 5'-triphosphate was not a substrate in the forward reaction, but would replace ADP and ATP in the reverse reaction. The reverse reaction was also used to make beta,gamma-imidoadenylyl 5'-tetraphosphate.

摘要

相似文献

1
Inhibition of T4 polynucleotide kinase by the ATP analog, beta, gamma-imidoadenylyl 5'-triphosphate.
Biochim Biophys Acta. 1978 Aug 7;525(2):357-63. doi: 10.1016/0005-2744(78)90230-9.
2
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Polynucleotide kinase.多核苷酸激酶
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Kinetic studies on the reaction catalyzed by polynucleotide kinase from phage T4-infected Escherichia coli.对噬菌体T4感染的大肠杆菌中多核苷酸激酶催化反应的动力学研究。
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Recognition of DNA substrates by T4 bacteriophage polynucleotide kinase.T4噬菌体多核苷酸激酶对DNA底物的识别
Nucleic Acids Res. 2004 Jan 30;32(2):653-60. doi: 10.1093/nar/gkh212. Print 2004.