Wessig Carsten
Department of Neurology, University of Würzburg, Würzburg, Germany.
Methods Mol Biol. 2011;686:267-71. doi: 10.1007/978-1-60761-938-3_12.
The blood-nerve barrier (BNB) separates the endoneurium from the endovascular space and the epineurial connective tissue. An intact BNB is very important for integrity and functions of the nerve fibers within the endoneurial space. Disruption of the BNB which leads to functional and structural impairment of the peripheral nerve plays an important role in many disorders of the peripheral nerve like Wallerian degeneration, inflammatory nerve disorders, and demyelination. So far, this increased BNB permeability can only be assessed ex vivo. Assessing BNB disruption in vivo would be of great value for studying disorders of the peripheral nervous system. Gadofluorine M (Gf), a new amphiphilic contrast agent for MRI, accumulates in rat nerves with increased permeability of the BNB. After application of Gf, T1-weighted MR images show contrast enhancement of nerves with a disrupted BNB. This new tool of assessing BNB permeability in vivo is described.
血-神经屏障（BNB）将神经内膜与血管内空间及神经外膜结缔组织分隔开来。完整的血-神经屏障对于神经内膜间隙内神经纤维的完整性和功能非常重要。血-神经屏障的破坏会导致周围神经的功能和结构受损，在许多周围神经疾病如沃勒变性、炎性神经疾病和脱髓鞘中起重要作用。到目前为止，这种血-神经屏障通透性增加只能在体外进行评估。在体内评估血-神经屏障的破坏对于研究周围神经系统疾病具有重要价值。钆双胺（Gf）是一种用于磁共振成像（MRI）的新型两亲性造影剂，会在血-神经屏障通透性增加的大鼠神经中蓄积。应用Gf后，T1加权磁共振图像显示血-神经屏障破坏的神经有对比增强。本文描述了这种在体内评估血-神经屏障通透性的新工具。
