血脑屏障破坏在脑肿瘤治疗中的应用

Blood-brain barrier disruption in the treatment of brain tumors.

作者信息

Blanchette Marie, Fortin David

机构信息

Department of Neurosurgery, University of Sherbrooke Hospital, Sherbrooke, QC, Canada.

出版信息

Methods Mol Biol. 2011;686:447-63. doi: 10.1007/978-1-60761-938-3_23.

DOI:10.1007/978-1-60761-938-3_23

PMID:21082387

Abstract

Standard chemotherapy administered systemically has a limited efficacy in the treatment of brain tumors. One of the major obstacles in the treatment of brain neoplasias is the impediment to delivery across the intact blood-brain barrier (BBB). Many innovative approaches have been developed to circumvent this obstacle. One such strategy is BBB disruption (BBBD), which successfully increases the delivery of antineoplastic agents to the central nervous system (CNS). This chapter describes the application of the BBBD technique in rats. Different methods to evaluate and measure BBB permeability following hyperosmolar mannitol infusion including Evans blue staining, albumin immunohistochemistry, and dynamic magnetic resonance imaging are also described.

摘要

全身给予的标准化疗在脑肿瘤治疗中的疗效有限。治疗脑肿瘤的主要障碍之一是完整血脑屏障（BBB）对药物递送的阻碍。人们已经开发出许多创新方法来克服这一障碍。一种这样的策略是血脑屏障破坏（BBBD），它成功地增加了抗肿瘤药物向中枢神经系统（CNS）的递送。本章描述了BBBD技术在大鼠中的应用。还介绍了在高渗甘露醇输注后评估和测量血脑屏障通透性的不同方法，包括伊文思蓝染色、白蛋白免疫组织化学和动态磁共振成像。