Ruiz-Ángel María Jose, Carda-Broch Samuel, García-Álvarez-Coque Maria Celia
Departament de Química Analítica, Universitat de València, c/Dr. Moliner 50, 46100, Burjassot, Spain.
Bioanalysis. 2009 Oct;1(7):1225-41. doi: 10.4155/bio.09.97.
The issue of doping control in sport involves the development of reliable analytical procedures and efficient strategies to process a large number of samples in a short period of time. Reversed-phase LC techniques with aqueous-organic mobile phases and MS or diode-array detection yield satisfactory results for the identification of prohibited substances in sport. However, time-consuming sample pretreatment steps are required, which reduces sample throughput. Micellar LC (MLC) that uses hybrid mobile phases of surfactant above its critical micellar concentration and organic solvent has been revealed as an interesting alternative. The surfactant sodium dodecyl sulfate solubilizes the protein components of urine, serum and plasma, which permits their direct injection into the chromatographic system. Only dilution and filtering of the samples may be required. Most MLC analyses are performed in isocratic mode, with short retention times and good selectivity. The sensitivity of MLC allows the detection of a variety of doping substances at least 24-48 h after being administered.
体育赛事中的兴奋剂检测问题涉及到开发可靠的分析程序和高效的策略，以便在短时间内处理大量样本。采用水-有机流动相的反相液相色谱技术与质谱或二极管阵列检测相结合，在识别体育赛事中违禁物质方面能产生令人满意的结果。然而，这需要耗时的样品预处理步骤，从而降低了样品通量。使用高于临界胶束浓度的表面活性剂与有机溶剂的混合流动相的胶束液相色谱（MLC）已被证明是一种有趣的替代方法。表面活性剂十二烷基硫酸钠可溶解尿液、血清和血浆中的蛋白质成分，从而允许将它们直接注入色谱系统。可能仅需对样品进行稀释和过滤。大多数MLC分析以等度模式进行，保留时间短且选择性好。MLC的灵敏度使得能够在施用各种兴奋剂物质后至少24 - 48小时检测到它们。
