Thomas David P, Foley Joe P
Analytical Operations, GPSG, A Division of Ortho-McNeil-Janssen Pharmaceuticals Inc., 1125 Trenton-Harbourton Road, Titusville, NJ 08560, USA.
Bioanalysis. 2010 Feb;2(2):229-35. doi: 10.4155/bio.09.171.
Detection of prohibited substances in equestrian sports typically involves time-consuming and tedious sample-preparation methods. Micellar LC (MLC) allows for direct injection of equine serum to detect prohibited NSAIDs.
The method was linear over the range of standards examined, with recoveries of 94.2-95.1% for phenylbutazone (12-18 µg/ml), and 83.9-88.7% and 87.9-105.0% for diclofenac and flunixin, respectively (0.1-1.2 µg/ml). The limit of detection was 0.1 µg/ml for all compounds and the limit of quantitation was 0.2 µg/ml for phenylbutazone and 0.3 µg/ml for diclofenac and flunixin.
The MLC method of direct equine serum injection was shown to be valid and simple for the quantitation of banned NSAIDs in equine serum.
马术运动中违禁物质的检测通常涉及耗时且繁琐的样品制备方法。胶束液相色谱法（MLC）可直接进样马血清以检测违禁的非甾体抗炎药。
在所检测的标准范围内该方法呈线性，保泰松（12 - 18微克/毫升）的回收率为94.2 - 95.1%，双氯芬酸和氟尼辛的回收率分别为83.9 - 88.7%和87.9 - 105.0%（0.1 - 1.2微克/毫升）。所有化合物的检测限均为0.1微克/毫升，保泰松的定量限为0.2微克/毫升，双氯芬酸和氟尼辛的定量限为0.3微克/毫升。
直接进样马血清的MLC方法被证明对于定量马血清中禁用的非甾体抗炎药是有效且简便的。
