Kirsch Stephan, Bindila Laura
Institute for Medical Physics and Biophysics, University of Münster, Robert Koch Straβe 31, D-48149 Münster, Germany.
Bioanalysis. 2009 Oct;1(7):1307-27. doi: 10.4155/bio.09.110.
In less than 5 years, an impressive number of methods based on nano-LC and HPLC-chip coupled online to MS were developed and implemented to comprehensively address structural heterogeneity of glycoconjugates and glycans in biological matrices. C18, graphitized carbon and amide-based stationary phases were adapted to nanoflow level and on chip format, leading to improved sensitivity of structural analysis and superior level of information on highly complex glycan and glycoconjugate mixtures. This review offers a summary of the recent progress in the application of nano-LC and HPLC-chip-MS in glycoanalytics of glycopeptides, glycoprotein glycans, glycosaminoglycans, oligosaccharides and glycosphingolipids.
在不到5年的时间里，人们开发并应用了大量基于纳升液相色谱（nano-LC）和与质谱（MS）在线联用的HPLC芯片的方法，以全面研究生物基质中糖缀合物和聚糖的结构异质性。C18、石墨化碳和酰胺基固定相被应用于纳流水平和芯片形式，从而提高了结构分析的灵敏度，并为高度复杂的聚糖和糖缀合物混合物提供了更高级别的信息。本文综述了nano-LC和HPLC芯片-MS在糖肽、糖蛋白聚糖、糖胺聚糖、寡糖和糖鞘脂糖分析中的应用进展。
