Schlotterbeck Götz, Ceccarelli Simona M
F Hoffmann-La Roche Ltd, Pharmaceuticals Division, CH-4070 Basel, Switzerland.
Bioanalysis. 2009 Jun;1(3):549-59. doi: 10.4155/bio.09.50.
Liquid chromatography (LC)-solid-phase extraction (SPE)-nuclear magnetic resonance (NMR)-mass spectrometry (MS) coupling is a key technology for fast and thorough structure elucidation of valuable mass-limited samples. Laborious serial isolation and purification procedures of metabolites, byproducts or impurities from complex biomatrices, natural product extracts or other mixtures of several components can be circumvented by the use of this integrated modular system. This combination of high-end analytical technology significantly accelerates the structure-elucidation process for valuable samples present in minute quantities in mixtures. The information depth is significantly increased by the concurrent availability of NMR and MS data of one chromatographic peak. Thus, this flexible technique is well on its way to becoming the gold standard in analytical chemistry of mixtures. LC-SPE-NMR-MS overcomes the limitations of directly coupled LC-NMR. Full flexibility regarding chromatographic conditions and NMR acquisition is gained by this modular technique. LC-SPE-NMR-MS allows for a rapid structure-elucidation process that would not be possible on the basis of MS or NMR data alone.
液相色谱（LC）-固相萃取（SPE）-核磁共振（NMR）-质谱（MS）联用技术是对珍贵的微量样品进行快速、全面结构解析的关键技术。通过使用这种集成模块化系统，可以避免从复杂生物基质、天然产物提取物或其他多组分混合物中费力地对代谢物、副产物或杂质进行一系列分离和纯化程序。这种高端分析技术的结合显著加快了混合物中微量存在的珍贵样品的结构解析过程。通过一个色谱峰的NMR和MS数据同时可得，信息深度显著增加。因此，这种灵活的技术正朝着成为混合物分析化学的金标准迈进。LC-SPE-NMR-MS克服了直接联用LC-NMR的局限性。这种模块化技术在色谱条件和NMR采集方面具有完全的灵活性。LC-SPE-NMR-MS能够实现仅基于MS或NMR数据无法完成的快速结构解析过程。
