液相色谱-串联质谱法测定人血浆中美洛昔康及其代谢物5-羧基美洛昔康
Liquid chromatography-tandem mass spectrometry method for the determination of meloxicam and its metabolite 5-carboxymeloxicam in human plasma.
作者信息
Lee Hye Won, Ji Hye Young, Kim Hoi Yun, Lee Kang Choon, Lee Hye Suk
机构信息
Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy, Wonkwang University, Iksan 570-749, Korea.
出版信息
Bioanalysis. 2009 Apr;1(1):63-70. doi: 10.4155/bio.09.10.
BACKGROUND
To develop and validate a rapid, sensitive and selective liquid chromatography-electrospray ionization mass spectrometric method for the determination of meloxicam and its metabolite 5-carboxymeloxicam in human plasma.
RESULTS
A liquid extraction method was chosen for sample clean-up. Meloxicam, 5-carboxymeloxicam and isoxicam (internal standard) were analyzed on an XBridge™ C18 column with 65% methanol in 10 mM ammonium formate (pH 3.0) and detected in selected reaction monitoring mode. The standard curves were linear over the concentration range 10-2500 ng/ml for meloxicam and 2-100 ng/ml for 5-carboxymeloxicam. Matrix effects were practically absent.
CONCLUSIONS
This method has been successfully applied to the pharmacokinetic study of meloxicam and 5-carboxymeloxicam after oral administration of meloxicam (15 mg) to 30 volunteers.
背景
建立并验证一种快速、灵敏且具选择性的液相色谱 - 电喷雾电离质谱法，用于测定人血浆中的美洛昔康及其代谢物5 - 羧基美洛昔康。
结果
选择液液萃取法进行样品净化。美洛昔康、5 - 羧基美洛昔康和异恶酰胺（内标）在XBridge™ C18柱上进行分析，流动相为含65%甲醇的10 mM甲酸铵（pH 3.0），采用选择反应监测模式进行检测。美洛昔康在10 - 2500 ng/ml浓度范围内、5 - 羧基美洛昔康在2 - 100 ng/ml浓度范围内标准曲线呈线性。几乎不存在基质效应。
结论
该方法已成功应用于30名志愿者口服美洛昔康（15 mg）后美洛昔康和5 - 羧基美洛昔康的药代动力学研究。
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