Lahaie Mathieu, Mess Jean-Nicholas, Furtado Milton, Garofolo Fabio
Algorithme Pharma Inc., Laval (Montreal), Quebec, Canada.
Bioanalysis. 2010 Jun;2(6):1011-21. doi: 10.4155/bio.10.65.
The objective is to find elution conditions using different solid-phase extraction chemistries to reduce the amount of phospholipids in plasma extract, which is associated with matrix effect in LC-MS.
Phospholipids' recovery was reduced by decreasing the eluents' methanol content applied to silica-based and polymer-based reversed-phase solid-phase extraction cartridges while 100% acetonitrile applied to the silica-based cartridges drastically minimized the phospholipids' elution. Silanol interactions are involved in the increased retention of phospholipids with silica-based reversed-phase cartridges when using high percentages of ACN in eluents. The bioanalytical usefulness of these findings was confirmed by successful extraction recovery of pharmaceutical compounds.
Combinations of specific eluents and reversed-phase solid-phase extraction cartridges were found to prevent matrix effect due to phospholipids.
目的是使用不同的固相萃取化学方法找到洗脱条件，以减少血浆提取物中磷脂的含量，这与液相色谱-质谱联用中的基质效应相关。
通过降低应用于硅胶基和聚合物基反相固相萃取柱的洗脱液中甲醇的含量，磷脂的回收率降低，而应用于硅胶基柱的100%乙腈极大地减少了磷脂的洗脱。当在洗脱液中使用高百分比的乙腈时，硅醇相互作用参与了磷脂在硅胶基反相柱上保留增加的过程。药物化合物的成功萃取回收率证实了这些发现的生物分析实用性。
发现特定洗脱液和反相固相萃取柱的组合可防止磷脂引起的基质效应。
