Vyas Chandni A, Flanigan Paul M, Shackman Jonathan G
Temple University, Dept of Chemistry, 1901 N. 13th Street, Philadelphia, PA 19122, USA.
Bioanalysis. 2010 Apr;2(4):815-27. doi: 10.4155/bio.10.17.
CE has evolved as one of the most efficient separation techniques for a wide range of analytes, from small molecules to large proteins. Modern microdevices facilitate integration of multiple sample-handling steps, from preparation to separation and detection, and often rely on CE for separations. However, CE frequently requires complex geometries for performing sample injections and maintaining zone profiles across long separation lengths in microdevices. Two novel methods for performing electrophoretic separations, gradient elution moving boundary electrophoresis (GEMBE) and gradient elution isotachophoresis (GEITP), have been developed to simplify microcolumn operations. Both techniques use variable hydrodynamic counterflow and continuous sample injection to perform analyses in short, simple microcolumns. These properties result in instruments and microdevices that have minimal 'real-world' interfaces and reduced footprints. Additionally, GEITP is a rapid enrichment technique that addresses sensitivity issues in CE and microchips.
毛细管电泳已发展成为一种针对从小分子到大型蛋白质等多种分析物的最有效分离技术之一。现代微型设备便于集成从样品制备到分离和检测的多个样品处理步骤，并且常常依靠毛细管电泳进行分离。然而，在微型设备中，毛细管电泳经常需要复杂的几何结构来进行样品进样以及在长分离长度上维持区带轮廓。已经开发出两种用于进行电泳分离的新方法，即梯度洗脱移动边界电泳（GEMBE）和梯度洗脱等速电泳（GEITP），以简化微柱操作。这两种技术都使用可变的流体动力逆流和连续样品进样，以便在短而简单的微柱中进行分析。这些特性使得仪器和微型设备具有最少的“实际”接口并减小了占地面积。此外，GEITP是一种快速富集技术，可解决毛细管电泳和微芯片中的灵敏度问题。
