Wu Dianna Y, Patti-Diaz Lisa, Hill Carla G
Bristol-Myers Squibb Company, 311 Pennington-Rocky Hill Road, Mail Stop 3B-201, Pennington, NJ 08534, USA.
Bioanalysis. 2010 Sep;2(9):1617-26. doi: 10.4155/bio.10.33.
Flow cytometry is a powerful analytical tool for the analysis of multiple biological parameters of individual cells or particles within heterogeneous cell populations. It has been widely used in biomedical research to perform immunophenotyping, cell counting and numerous cell function assessments, such as intracellular cytokine production, protein phosphorylation, cell proliferation and apoptosis. The implementation of standardized flow cytometry-based biomarker assays in clinical trials remains a challenge due to the limited stability of clinical specimens and the technical variations between instruments. To ensure data quality, it is crucial to develop robust assays for clinical applications. In this review, we summarize current practice in developing, validating and implementing flow cytometry assays to evaluate biomarkers in clinical research.
流式细胞术是一种强大的分析工具，用于分析异质细胞群体中单个细胞或颗粒的多种生物学参数。它已广泛应用于生物医学研究，以进行免疫表型分析、细胞计数和众多细胞功能评估，如细胞内细胞因子产生、蛋白质磷酸化、细胞增殖和凋亡。由于临床样本稳定性有限以及仪器之间的技术差异，在临床试验中实施基于流式细胞术的标准化生物标志物检测仍然是一项挑战。为确保数据质量，开发适用于临床应用的可靠检测方法至关重要。在本综述中，我们总结了目前在开发、验证和实施流式细胞术检测以评估临床研究中的生物标志物方面的实践。
