Villas B H
Hematology Laboratory, University of Florida Health Science Center, Jacksonville, USA.
Cell Vis. 1998 Jan-Feb;5(1):56-61.
Flow cytometry is an innovative technology that measures certain cell parameters as the cells flow in a fluid stream and in single file past an analytical laser light source. Clinical applications of flow cytometry currently utilized in the laboratory include cell surface antigen determinations or immunophenotyping of hematologic cells, DNA analysis of hematopoietic malignancies and solid tumors, and measurement of CD4 (T helper/inducer cell) absolute counts and T helper/T suppressor (CD4/CD8) ratios in the evaluation of immune deficiency. Flow cytometry often offers a more rapid, sensitive, accurate, and quantitative means of analyzing a particular cell population in a heterogeneous cell suspension as compared to more traditional microscopic methods. This article is intended to provide a general understanding of the technological basis of how a flow cytometer functions as well as an overview of both current and future flow cytometric clinical laboratory applications.
流式细胞术是一项创新技术，当细胞在液流中单个排列通过分析激光光源时，它能测量某些细胞参数。目前实验室中使用的流式细胞术的临床应用包括血细胞的细胞表面抗原测定或免疫表型分析、造血系统恶性肿瘤和实体瘤的DNA分析，以及在免疫缺陷评估中测量CD4（T辅助/诱导细胞）绝对计数和T辅助/T抑制（CD4/CD8）比值。与更传统的显微镜方法相比，流式细胞术通常能提供一种更快速、灵敏、准确且定量的手段来分析异质性细胞悬液中的特定细胞群体。本文旨在让大家对流式细胞仪的工作技术基础有一个总体了解，并概述流式细胞术在临床实验室当前及未来的应用。
