Department of Life Sciences, Imperial College London, Exhibition Road, London SW72AZ, UK.
Protein Eng Des Sel. 2011 Jan;24(1-2):229-31. doi: 10.1093/protein/gzq102. Epub 2010 Nov 17.
Rapid determination of the three-dimensional structures of mutant proteins is so highly embedded within the discipline of protein engineering that it almost seems a routine part of the process of analyzing mutant function. However, in the early days of protein engineering, the time and energy involved in determining mutant structures were substantial compared with today and the dividends unclear. Here is a brief perspective on the determination of the first crystal structures of site-directed mutants of the tyrosyl-tRNA synthetase--an enzyme used to establish many of the early principles of protein engineering.
快速测定突变蛋白的三维结构在蛋白质工程学科中已经非常成熟，几乎成为分析突变体功能过程中的常规步骤。然而，在蛋白质工程的早期，与今天相比，测定突变体结构所涉及的时间和精力都相当大，而收益却并不明确。本文简要介绍了用于确定酪氨酸 tRNA 合成酶的定点突变体的第一个晶体结构的情况，该酶用于确定许多早期蛋白质工程的原理。
